The capsule can be visualized by several microscopy techniques but in pneumococci the presence of a capsular polysaccharide is usually detected using specific sera [1,12]. A positive result is reported if a zone radius (on a 6 mm disc) is >8 mm. Serotyping was clinically relevant in the preantibiotic 1920s to 1930s, when capsule-specific horse antisera were administered for therapy. This exchange of genetic information occurs under experimental conditions and, more importantly, also in nature.15 Thus, in addition to a highly conserved genetic core, S. pneumoniae supports a large number of noncore genes that provide remarkable diversity of genetic loci between isolates, particularly related to antimicrobial targets and targets of immune recognition (capsular polysaccharides and adhesins).16,17, Lisa A. Jackson, in Vaccines (Sixth Edition), 2013. Information and translations of optochin in the most comprehensive dictionary definitions resource on … Genetic control of this complex set of events has been elucidated for some serotypes; for example, a cassette of 15 genes that function as a single transcriptional unit is responsible for encapsulation in serogroup 19.12 More than 92 serotypes of S. pneumoniae have been identified on the basis of antigenic differences in their capsular polysaccharides. However, since the production of a capsular polysaccharide is such a defining trait of pneumococci, these have been subject to particularly stringent tests to confirm their identification as S. pneumoniae [13]. Pneumolysin triggers different proinflammatory reactions and can activate the classical complement pathway (Boulnois et al., 1991; Houldsworth et al., 1994). Streptococcus pneumoniae strains are sensitive to the chemical optochin (ethylhydrocupreine hydrochloride). Optochin sensitivity can be used in primary plates from specimens (e.g. Edward N. Janoff, Daniel M. Musher, in Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases (Eighth Edition), 2015. An important property of S. pneumoniae is its capacity, as part of its quorum-sensing mechanism, to express a competence-sensing protein and internalize DNA from other pneumococci or from other bacterial species.14 This horizontal transfer of genetic information to pneumococci, called transformation, enables pneumococci to acquire new traits. Further factors supporting nasopharyngeal colonization include neuraminidase NanA and immunoglobulin (Ig) A protease. The Quellung reaction is, however, not sufficiently sensitive to distinguish serotypes with small structural differences, and more recently, molecular techniques have been used to identify new pneumococcal serotypes, such as 6 C, which was indistinguishable from serotype 6A using the Quellung reaction.23 Antipneumococcal vaccines are based on formulations of various capsular (polysaccharide) antigens derived from the selected serotypes. What is the test’s principle? Recently, it was argued that this distinction could be made possible by a more detailed analysis of the mass profiles [9] and this was followed by a publication reporting the success of a commercially available system in distinguishing S. pneumoniae from other species of the mitis group [11]. Ethylhydrocupreine hydrochloride (optochin), is a quinine derivative. Copyright © 2020 Elsevier B.V. or its licensors or contributors. Serovars of pneumococci are differentiated according to their capsular polysaccharide content, and serovars 4 and 19 are the two most often recovered from guinea pigs (Fox, 2002; Percy and Barthold, 2007). Optochin is an antibiotic that interferes with the ATPase and production of adenosine triphosphate (ATP) in microorganisms. Monitoring for S. pneumoniae infection is often conducted by nasopharyngeal culture onto blood agar. The Statens Serum Institut in Copenhagen, Denmark is the most frequent source of sera to identify pneumococcal capsules. What does optochin mean? Gillespie MB, BCh, BAO, MRCP(UK), MRCPath, in Medical Microbiology Illustrated, 1994. Meaning of optochin. This site uses Akismet to reduce spam. A 5 μg impregnated disc is used. S. pneumoniae is a gram-positive coccus that replicates in chains in liquid medium but appears as lancet-shaped diplococci in clinical specimens. Among the multiple genes that encode production of individual capsules, some are specific for individual polysaccharides, whereas others are conserved among nearly all pneumococci and even some other streptococci.13. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. URL:, URL:, URL:, URL:, URL:, URL:, URL:, URL:, URL:, URL:, Tropical Infectious Diseases (Third Edition), The pneumococcus is a Gram-positive coccus that is usually identified on the basis of, Bacterial Infections of the Central Nervous System, is a gram-positive, α-hemolytic, catalase-negative, and, Boulnois et al., 1991; Houldsworth et al., 1994, Glen M. Otto DVM, DACLAM, ... Charles B. Clifford DVM, PhD, DACVP, in, Laboratory Animal Medicine (Third Edition), from other α-hemolytic streptococci is most often performed by the, Principles and Practice of Pediatric Infectious Diseases (Fourth Edition), , α-hemolysis on blood agar and susceptible to, The Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents, is an α-hemolytic streptococcus that ferments trehalose and is, Harkness and Wagner, 1995; Keyhani and Naghshineh, 1974; Songer and Post, 2005, Bacteriology of the Head and Neck Regions, ). Immunization of rabbits with a pneumococcus of a specific capsular type stimulates the appearance of antibodies that cause agglutination and create a hydrophobic border around the capsule. The identification of S. pneumoniae from culture depends on the observation of the morphologic characteristics discussed above and on three other main phenotypic characteristics, namely catalase negativity, optochin susceptibility and bile solubility. Pneumococci are relatively fastidious, facultative anaerobic organisms that grow in short chains in broth culture and appear as lancet-shaped gram-positive diplococci or chains of cocci when examined microscopically (Figure 26-1). 201-1). Blogging is my passion. The tubes are incubated at 30°C and a positive result is reported if clearing of turbidity occurs. Adherence of pneumococci to mammalian cells is affected by phase variations, in which colonial morphology of the pathogen shifts from opaque to transparent (Fig. Glen M. Otto DVM, DACLAM, ... Charles B. Clifford DVM, PhD, DACVP, in Laboratory Animal Medicine (Third Edition), 2015. Pneumolysin is important for pneumococcal respiratory infections and invasion into the bloodstream. Optochin susceptibility is a presumptive test only. How do you use optochin in a sentence? Traditionally, capsular serotypes were defined by the Quellung reaction, using polyclonal rabbit sera. The original observations of bacteria that we now believe were Streptococcus pneumoniae are attributed to Klebs (1875), Eberth and Mátray (1880), the latter having coined the term Pneumoniekokken [1].

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